ABSTRACT
BACKGROUND@#Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. @*METHODS@#An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n= 14). The DHP scaffold, 1 x 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. @*Results@#The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p 0.05). @*Conclusion@#In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
ABSTRACT
BACKGROUND@#Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. @*METHODS@#An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n= 14). The DHP scaffold, 1 x 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. @*Results@#The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p 0.05). @*Conclusion@#In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
ABSTRACT
Milky spots in the human omental tissue are known to be consisting of lymphocytes, macrophages and mast cells. Our goal was to evaluate the relationship of lymphoid cells and macrophages with vasculature and stromal components. In this study we examined the biopsy specimens obtained from the adult patients whom were operated for different purposes in the General Surgery Department of Dicle University Hospital, Ankara, Turkey. We used CD31 as an endothelial cell marker, CD36 which is known to react with microvascular endothelium and adipocytes, and CD44 which is a hyaluronic acid receptor using an indirect immunoperoxidase technique. We observed that CD31 was mainly reactive with vascular endothelial cells and platelets, CD36 was reactive with microvascular endothelium and adipocytes and CD44 was mainly expressed by the endothelial cells of high endothelial venules, fibroblasts in stromal compartments and by large mononuclear cells. We determined the structural and immunophenotypic features of omental lymphoid tissue components stressing vascular and stromal elements, and we briefly discussed the significance of the expression of these molecules in the determined locations
Subject(s)
Humans , Immunohistochemistry , Platelet Endothelial Cell Adhesion Molecule-1/isolation & purification , CD36 Antigens/isolation & purification , Hyaluronan Receptors/isolation & purification , Clinical Laboratory TechniquesABSTRACT
To present additional data on high endothelial venule [HEV] structure and immunophenotype. We used the zinc iodide-osmium tetroxide technique [ZIO], which is a metallophilic fixation and staining technique to examine HEVs at light and electron microscopic levels as this technique was previously reported to be reactive with cells in HEVs. Tonsils and lymph nodes were obtained from the Surgery and Otorhinolaryngology Departments, Hacettepe University Hospital, Ankara, Turkey during 2002 and 2003. An indirect immunohistochemical technique was used to examine frozen human tissue samples. Organelle rich high endothelial cells, sheet-like processes of pericytes surrounding HEVs, structural relation of pericyte processes with fibroblastic reticular cells, an unusual multivesicular body-like organelle within high endothelial cells were presented. Expression of a large panel of defined and yet non-defined antigens on HEVs are also presented using an indirect immunoperoxidase technique. Presence of some of these antigens on HEVs was previously reported while no previous report is available for others. Significance of the expression of these antigens in HEVs, structural hints for trans endothelial migration of lymphocytes and their travel along the reticular cell meshwork is briefly discussed